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The image shows a diagram depicting the steps of isolating a gene by subtractive hybridization. The steps are: shear and biotinylate DNA, denature, reassociate, bind to avidin-coated beads, unbind DNA, PCR amplification, ligate to vector and transform bacteria. The diagram includes arrows indicating the direction of the process. The diagram is printed on a page of a book, which also includes text discussing the process of gene cloning. The text mentions yeast as a cloning host and the benefits of using yeast for cloning. The text discusses the importance of using the correct host cell for cloning. The text mentions that the process of cloning is often used to identify genes involved in specific cellular processes. The page is likely from a textbook or scientific journal.
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